Thermo Fisher Scientific CD16b Monoclonal Antibody (8F1C10)
Human CD16b 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, Flow cytometry, ELISA에 적합. Protein G로 정제된 액상형 제품이며 1 mg/mL 농도로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8F1C10 |
| Immunogen | Purified recombinant fragment of human CD16B (AA: 17–200) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090741 |
Target Information
CD16 (FCGR3A) is a 50–65 kDa cell surface molecule that exists in two forms:
- A transmembranous form expressed by NK cells and some T cells
- A phosphatidylinositol-linked form expressed by granulocytes
CD16 is a low-affinity receptor for IgG (FcR III) and plays a key role in mediating antibody-dependent cellular cytotoxicity (ADCC) by NK cells.
Human CD16 is expressed in two forms, FCGR3A and FCGR3B:
- FCGR3A associates with the FcepsilonRI-gamma subunit and mediates NK cell cytotoxicity.
- FCGR3B is a GPI-linked receptor expressed on neutrophils, involved in activation and induction of a pro-adhesive phenotype.
Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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