
Thermo Fisher Scientific HDAC9 Recombinant Rabbit Monoclonal Antibody (JU30-44)
HDAC9 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, IP에 적합합니다. HEK293 세포에서 발현되며 높은 특이성과 재현성을 제공합니다. 단백질 A로 정제되었으며, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | 1:10–1:50 | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU30-44 |
| Immunogen | Synthetic peptide within Human HDAC9 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810096 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Within the cell, DNA associates with histones and other nuclear proteins to form chromatin. Chromatin remodeling is essential for transcriptional regulation, and histone acetylation plays a major role in this process by increasing DNA accessibility to transcription factors.
Histone deacetylases (HDACs), including HDAC1–9, reverse this acetylation and are associated with transcriptional silencing. HDAC9 and HDAC9a are alternatively spliced isoforms; HDAC9a is 132 amino acids shorter but retains catalytic activity. Both isoforms repress myocyte enhancer-binding factor 2-mediated transcription and are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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