Thermo Fisher Scientific TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (TNFA, 1172)
인간 및 랫트 TNF-alpha 단백질을 인식하는 단클론 항체로, IHC, ICC/IF, Flow Cytometry에 적합합니다. Protein A/G로 정제된 액상 항체이며, 4°C에서 보관합니다. 면역조직화학에서 고온 Tris-EDTA 전처리로 최적 결과를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TNFA, 1172 |
| Immunogen | Recombinant full-length human TNF-alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature (RT).
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.
Target Information
TNF alpha (Tumor Necrosis Factor alpha) is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers but shows a single 17 kDa band on SDS-PAGE under non-reducing conditions. Knockout studies indicate its neuroprotective and tumor necrosis functions. TNF alpha plays roles in immune responses to bacterial, viral, parasitic, and fungal infections and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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