Thermo Fisher Scientific POU6F1 Polyclonal Antibody
Human POU6F1 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. PBS 기반 저장용액에 20% 글리세롤 포함, 4°C 단기 또는 -20°C 장기 보관 권장.
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- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–301 of Human POU6F1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545702 |
Product Specific Information
- Recommended positive controls: A549, H1299, HCT116
- Predicted reactivity: Mouse (94%), Rat (96%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
Brn-5 (Brain-5), also known as POU6F1, MPOU, or TCFB1, is a widely expressed protein but during embryogenesis is exclusively found in the developing brain and spinal cord.
As is characteristic of Brn family members, Brn-5 contains two DNA-binding domains—the POU-specific domain and the POU homeodomain—each with an HTH (helix-turn-helix) motif.
Brn-5 binds to CRH (corticotrophin-releasing hormone) elements with high affinity and can enhance Prolactin gene expression and activate Pit-1 expression.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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