Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), APC, eBioscience

Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), APC, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• Unconjugated
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family involved in inhibiting immune responses. HLA-G has seven reported isoforms, and the 87G antibody recognizes both HLA-G1 and soluble HLA-G5.

HLA-G expression is primarily found in fetal trophoblast cells invading the maternal decidua, protecting the fetus from maternal immune attack. High expression has also been identified in certain tumors such as melanoma, breast carcinoma, renal carcinoma, CLL, AML, and B-CLL. Additional expression is observed in pancreatic islets, erythroid and endothelial progenitors, and the adult thymic medulla.

HLA-G⁺ CD4 or CD8 cells in normal human peripheral blood are thought to act as regulatory cells with a unique cytokine profile distinct from Tregs. The receptors for HLA-G include CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies indicate a role for HLA-G in transplant tolerance and maintenance.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on stimulated U937 cells
• Recommended Usage: 5 µL (0.125 µg) per 100 µL test volume
• Cell Range: 10⁵–10⁸ cells/test
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (β2-microglobulin). The heavy chain (~45 kDa) is membrane-anchored and encoded by a gene with 8 exons:

• Exon 1: Leader peptide
• Exons 2 & 3: α1 and α2 domains (peptide-binding)
• Exon 4: α3 domain
• Exon 5: Transmembrane region
• Exon 6: Cytoplasmic tail

HLA-G is expressed on fetal-derived placental cells and plays a key role in immune tolerance. HLA and MHC antibodies are important tools in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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