Thermo Fisher Scientific MHC I Free Chain Monoclonal Antibody (A4), APC, eBioscience

Thermo Fisher Scientific MHC I Free Chain Monoclonal Antibody (A4), APC, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin.
There are three class I alpha-chain genes in humans, called HLA-A, -B, and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (β2M).
The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen-specific CD8+ cytotoxic T cells.
Class I HLA is expressed by the majority of nucleated cells.

Applications Reported: This A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

There are three class I alpha-chain genes in humans, called HLA-A, -B, and -C. Normally, HLA class I proteins are associated non-covalently with β2 microglobulin (β2M).
The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen-specific CD8+ cytotoxic T cells.
Class I HLA is expressed by the majority of nucleated cells.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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