Thermo Fisher Scientific CD163 Monoclonal Antibody (OTI3D4), TrueMAB

Applications

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and a member of the scavenger receptor cysteine-rich superfamily. It functions as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and facilitating iron recycling via endocytosis and heme breakdown.

CD163 is expressed on human monocytes and macrophages, predominantly during the late phase of inflammation. It is found on all circulating monocytes and most tissue macrophages except those in mantle zones, germinal centers, interdigitating reticulum cells, and Langerhans cells. Expression is high on CD14⁺ and CD16⁺ monocytes and is upregulated by IL-10, IL-6, and dexamethasone. LPS and PMA induce shedding of CD163 into plasma or supernatant.

CD163 binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, with higher affinity for multimeric haptoglobin (HP1F phenotype) complexes. It also triggers intracellular signaling involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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