Thermo Fisher Scientific CD163 Monoclonal Antibody (OTI2B12), TrueMAB
인간 CD163 단백질을 인식하는 Mouse IgG2a 단클론 항체로 다양한 응용(WB, IHC, ICC, Luminex)에 사용 가능. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼 제공. 조직 및 세포 내 CD163 발현 연구에 적합.
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- CF506382
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Luminex (LUM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B12 |
| Immunogen | Full-length human recombinant protein of CD163 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily.
It serves as a receptor for the hemoglobin-haptoglobin complex and protects tissues from oxidative damage caused by free hemoglobin.
CD163 is expressed on human monocytes and macrophages, especially during the late phase of inflammation.
Expression is upregulated by IL-10, IL-6, and dexamethasone, while LPS and PMA induce shedding of CD163 into plasma or supernatant.
Binding to hemoglobin/haptoglobin complexes is calcium- and pH-dependent, with higher affinity for multimeric haptoglobin (HP1F phenotype).
CD163 also triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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