Thermo Fisher Scientific Actin Monoclonal Antibody (ACTN05 (C4))

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 342 publications 342 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

-

Immunocytochemistry (ICC/IF)

1:10-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg

View 6 publications 6 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Pig, Protozoa, Rabbit, Rat

Published species

C. elegans, Chordate, Dog, Ferret, Fish, Fruit fly, Hamster, Human, Insect, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Xenopus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ACTN05 (C4)

Immunogen

Chicken gizzard actin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Actin,uniProtId:P08023-1,ncbiNodeId:9031,antigenRange:1-377,antigenLength:377,antigenImageFileName:MA5-11869_Actin_P08023-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11869_Actin_P08023-1_House_mouse_PDP.jpeg,sortOrder:8}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

0.3 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11004139

Product Specific Information

MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.

Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.

MA5-11869 is a suggested replacement to Product # 82353, clone # C4.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.