Thermo Fisher Scientific Actin Monoclonal Antibody (ACTN05 (C4))
모든 6가지 척추동물 액틴 아이소폼에 반응하는 mouse monoclonal antibody. Western blot, IHC, ICC 등 다양한 응용 가능. 총 단백질 로딩 검출에 적합하며, HeLa 세포 및 골격근을 양성 대조로 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | 342 publications |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:200 | 3 publications |
| Immunoprecipitation (IP) | 2 µg | 6 publications |
| Immunomicroscopy (IM) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Pig, Protozoa, Rabbit, Rat |
| Published Species | C. elegans, Chordate, Dog, Ferret, Fish, Fruit fly, Hamster, Human, Insect, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Xenopus, Yeast, Zebrafish |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ACTN05 (C4) |
| Immunogen | Chicken gizzard actin (Actin, UniProt ID: P08023-1, antigen range 1–377, antigen length 377) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11004139 |
Additional Formats
Product Specific Information
MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42 kDa), including two cytoplasmic actins (beta and gamma) that differ from muscle actins by approximately 25 amino acid residues.
This antibody is highly recommended for monitoring total protein load on Western blots.
For formalin-fixed, paraffin-embedded tissues, boil sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, then cool at room temperature for 20 minutes.
Suggested positive controls: HeLa cells and skeletal muscle.
MA5-11869 is a suggested replacement for Product #82353, clone #C4.
Target Information
Actin is a ubiquitous protein forming filaments that constitute a major part of the cytoskeleton.
Actin filaments interact with myosin to facilitate muscle contraction, cell motility, and cytokinesis.
In vertebrates, actin isoforms are classified as alpha, beta, and gamma:
- Alpha actins: Found in muscle tissues, key in contractile apparatus
- Beta and gamma actins: Present in most cell types, mediating cytoskeletal organization and internal motility
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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