Invitrogen™

EnzChek™ Pyrophosphate Assay Kit

The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In자세히 알아보기

The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically.

See our complete line of Fluorescence Microplate assays.

• Sensitivity limit of 1 μM pyrophosphate (∼0.2 μg/mL)
• Acommodates a pH range of 6.5 to 8.5

The EnzChek™ Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters.

The EnzChek™ Pyrophosphate Assay Kit is a modification of our EnzChek™ Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek™ Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.

사양

색상Ultraviolet

검출 방법Colorimetric

용도(애플리케이션)Pyrophosphate Assay

용도 (장비)Spectrophotometer

제품 유형Pyrophosphate Assay

염료 유형MESG

형식Tube(s)

제품라인EnzChek™

수량100 assays

배송 조건Room Temperature

Unit SizeEach