Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, Biotin
염소 유래의 항-토끼 IgG (H+L) 2차 항체로 Biotin 결합형입니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 높은 특이성과 감도를 제공하며, Affinity chromatography로 정제되어 신뢰성 높은 결과를 제공합니다.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534774 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and exhibit high specificity for rabbit immunoglobulins. They are ideal for detection, sorting, or purification of rabbit IgG targets. Secondary antibodies enhance versatility and sensitivity through signal amplification, enabling use with various detection systems such as HRP, AP, and fluorescence. Typically, they are produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, conjugation).
Additional Formats Available
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Request custom conjugation available
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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