Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP
염색 효소 AP가 결합된 염소 유래 항-토끼 IgG(H+L) 2차 항체로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제, 높은 특이성과 감도로 신뢰성 있는 검출 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534773 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of antibody is validated for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and have well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit antibodies. Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- Biotin
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- HRP
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available on request
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