Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody
염소 유래 항-토끼 IgG (H+L) 2차 항체로, WB·ICC·ELISA 등 다양한 면역실험에 사용 가능. 고순도 Affinity Chromatography 정제. 비결합형 형태로 높은 특이성과 감도를 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:4000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534772 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of antibody is validated for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and exhibit well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies enhance versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and improve sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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