Thermo Fisher Scientific CX3CL1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Fractalkine (CX3CL1). Anti-Human Fractalkine (CX3CL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hFractalkine by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Fractalkine (500-P98) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hFractalkine.

Western Blot:
To detect hFractalkine by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hFractalkine is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P98BT-1MG will be provided as 2 × 500 µg.

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues.
CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively.
Recent studies show CX3CL1 can suppress production of nitrous oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting a role as an intrinsic inhibitor against neurotoxicity by activated microglia.
Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.