Thermo Fisher Scientific CX3CL1 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Fractalkine (CX3CL1) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CX3CL1,uniProtId:P78423-1,ncbiNodeId:9606,antigenRange:25-100,antigenLength:397,antigenImageFileName:500-P98BT-500UG_CX3CL1_P78423-1_Rabbit.svg,antigenImageFileNamePDP:500-P98BT-500UG_CX3CL1_P78423-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Fractalkine (CX3CL1). Anti-Human Fractalkine (CX3CL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hFractalkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Fractalkine (500-P98) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFractalkine.

Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P98BT-1MG will be provided as 2 x 500 µg

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.