Thermo Fisher Scientific CX3CL1 Polyclonal Antibody, PeproTech
CX3CL1(Fractalkine)에 특이적인 Rabbit Polyclonal Antibody로, Western Blot, ELISA, ICC/IF 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Human 시료에 반응. -20°C 보관, PBS 기반 완충액, 방부제 무첨가.
- 카탈로그번호
- 500-P98-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CX3CL1 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Fractalkine (CX3CL1) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930893 |
Product Specific Information
AA Sequence of recombinant protein:
QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Fractalkine (CX3CL1). The antibody was purified by affinity chromatography employing an immobilized Human Fractalkine (CX3CL1) matrix.
Sandwich ELISA:
To detect Human Fractalkine (CX3CL1) by sandwich ELISA (using 100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When used with PeproTech Biotinylated Anti-Human Fractalkine (CX3CL1) (500-P98Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human Fractalkine (CX3CL1).
Western Blot:
To detect hFractalkine by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hFractalkine is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Chemokines are a family of proteins associated with leukocyte trafficking in immune surveillance and inflammatory recruitment. CX3CL1 (Fractalkine) is a CX3C chemokine known to induce adhesion and migration of leukocytes via membrane-bound and soluble forms. It can suppress nitrous oxide, interleukin-6, and TNF-α production in activated microglia and neuronal cells, suggesting an inhibitory role in neurotoxicity. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and infection, which can be inhibited by CX3CL1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.