Thermo Fisher Scientific PRAME Monoclonal Antibody (CL5148)
인간 PRAME 단백질을 인식하는 마우스 단일클론 항체로, Western blot 및 IHC(P) 분석에 적합함. 고순도 Protein A 정제, 액상 형태로 제공되며 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL5148 |
| Immunogen | Recombinant protein corresponding to Human PRAME (UniProt ID: P78395-1, antigen range: 265–390) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2787045 |
Product Specific Information
Immunogen sequence:
LLLSHIHASS YISPEKEEQY IAQFTSQFLS LQCLQALYVD SLFFLRGRLD QLLRHVMNPL ETLSITNCRL SEGDVMHLSQ SPSVSQLSVL SLSGVMLTDV SPEPLQALLE RASATLQDLV FDECGI
Target Information
This gene encodes an antigen predominantly expressed in human melanomas and recognized by cytolytic T lymphocytes. It is not expressed in normal tissues except testis. This expression pattern is similar to other CT antigens such as MAGE, BAGE, and GAGE. Unlike these, PRAME is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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