Thermo Fisher Scientific MDS028 Monoclonal Antibody (3D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

3 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D4

Immunogen

MDS028 (AAH06552, 1 a.a. approximately 447 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ITFG2,uniProtId:Q969R8-1,ncbiNodeId:9606,antigenRange:1-447,antigenLength:447,antigenImageFileName:H00055846-M05_ITFG2_Q969R8-1_House_mouse.svg,antigenImageFileNamePDP:H00055846-M05_ITFG2_Q969R8-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MRSVSYVQRV ALEFSGSLFP HAICLGDVDN DTLNELVVGD TSGKVSVYKN DDSRPWLTCS CQGMLTCVGV GDVCNKGKNL LVAVSAEGWF HLFDLTPAKV LDASGHHETL IGEEQRPVFK QHIPANTKVM LISDIDGDGC RELVVGYTDR VVRAFRWEEL GEGPEHLTGQ LVSLKKWMLE GQVDSLSVTL GPLGLPELMV SQPGCAYAIL LCTWKKDTGS PPASEGPTDG SRETPAARDV VLHQTSGRIH NKNVSTHLIG NIKQGHGTES SGSGLFALCT LDGTLKLMEE MEEADKLLWS VQVDHQLFAL EKLDVTGNGH EEVVACAWDG QTYIIDHNRT VVRFQVDENI RAFCAGLYAC KEGRNSPCLV YVTFNQKIYV YWEVQLERME STNLVKLLET KPEYHSLLQE LGVDPDDLPV TRALLHQTLY HPDQPPQCAP SSLQDPT

Target Information

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.