Thermo Fisher Scientific E-Cadherin (CDH1)/CD324 Recombinant Mouse Monoclonal Antibody (rCDH1, 1525)
E-Cadherin(CDH1)/CD324 단백질을 인식하는 Thermo Fisher Scientific의 재조합 마우스 단일클론 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, HEK293 발현 시스템을 사용한 고순도 항체로 연구용으로 제공됩니다.
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Thermo Fisher Scientific E-Cadherin (CDH1)/CD324 Recombinant Mouse Monoclonal Antibody (rCDH1, 1525)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rCDH1, 1525 |
| Immunogen | Recombinant full-length human E-Cadherin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse or Rat.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (calcium-dependent adhesion protein) superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in CDH1 are associated with various cancers including gastric, breast, colorectal, thyroid, and ovarian cancer. Loss of E-Cadherin function contributes to tumor progression by promoting proliferation, invasion, and metastasis.
E-Cadherin plays a crucial role in maintaining tissue architecture and integrity, and is encoded by the CDH1 gene on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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