Thermo Fisher Scientific KPNB1 Monoclonal Antibody (OTI10B7), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Nucleocytoplasmic transport is a signal- and energy-dependent process that occurs through nuclear pore complexes in the nuclear envelope. Import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins).
Importin alpha binds the NLS-containing cargo in the cytoplasm, while importin beta mediates docking at the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP-binding protein Ran, the complex translocates through the pore, where importin subunits dissociate. Importin alpha enters the nucleoplasm with its cargo, and importin beta remains at the pore. Interactions between importin beta and FG repeats of nucleoporins are critical for translocation.
The KPNB1 gene encodes a member of the importin beta family involved in this transport mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.