Thermo Fisher Scientific Ku80 Recombinant Rabbit Monoclonal Antibody (ARC0706)
Ku80 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 인간 및 생쥐 시료에 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Ku80 Recombinant Rabbit Monoclonal Antibody (ARC0706)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0706 |
| Immunogen | Synthetic peptide corresponding to amino acids 633–732 of human Ku80 (P13010) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897764 |
Product Specific Information
- Positive Samples: HeLa, A-549, MCF7
- Immunogen Sequence:
MKSIDCIRAF REEAIKFSEE QRFNNFLKAL QEKVEIKQLN HFWEIVVQDG ITLITKEEAS GSSVTAEEAK KFLAPKDKPS GDTAAVFEEG GDVDDLLDMI
Target Information
Ku80 is the 80-kDa subunit of the Ku heterodimer protein, also known as ATP-dependent DNA helicase II or DNA repair protein XRCC5. It serves as the DNA-binding component of DNA-dependent protein kinase and functions with the DNA ligase IV–XRCC4 complex in the repair of DNA double-strand breaks by non-homologous end joining and in V(D)J recombination. This gene complements the Chinese hamster xrs-6 mutant defective in DNA repair and recombination. A rare microsatellite polymorphism in Ku80 is associated with cancer in patients exhibiting variable radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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