Thermo Fisher Scientific CD3e Recombinant Mouse Monoclonal Antibody (898H2-6-15)

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Assay-dependent

Flow Cytometry (Flow)

• Tested Dilution: Assay-dependent

Product Specifications

Immunogen

Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes.
Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.

Product Specific Information

Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically.
It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 antibody (STH164).
CD3, and the ε chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.

Target Information

The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits (γ, δ, ε, ζ).
These CD3 subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.
CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, mediated by a double tyrosine-based motif present in a single copy in the CD3 subunits.
CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum.
It is not expressed on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
The genes encoding CD3 epsilon, gamma, and delta polypeptides are located on chromosome 11.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: MA5-51799_CD3 관련 이미지들)