Thermo Fisher Scientific CD3e Recombinant Mouse Monoclonal Antibody (898H2-6-15)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Pig

Host/Isotype

Mouse / IgG2a, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

898H2-6-15

Immunogen

Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD3,uniProtId:Q7YRN2-1,ncbiNodeId:9821,antigenRange:1-196,antigenLength:196,antigenImageFileName:MA5-51799_CD3_Q7YRN2-1_House_mouse.svg,antigenImageFileNamePDP:MA5-51799_CD3_Q7YRN2-1_House_mouse_PDP.jpeg,sortOrder:10},{targetFamily:CD3,uniProtId:Q5PXD3-1,ncbiNodeId:9821,antigenRange:1-182,antigenLength:182,antigenImageFileName:MA5-51799_CD3_Q5PXD3-1_House_mouse.svg,antigenImageFileNamePDP:MA5-51799_CD3_Q5PXD3-1_House_mouse_PDP.jpeg,sortOrder:14},{targetFamily:CD3,uniProtId:Q764N2-1,ncbiNodeId:9821,antigenRange:1-171,antigenLength:171,antigenImageFileName:MA5-51799_CD3_Q764N2-1_House_mouse.svg,antigenImageFileNamePDP:MA5-51799_CD3_Q764N2-1_House_mouse_PDP.jpeg,sortOrder:15},{targetFamily:CD3,uniProtId:Q9XSJ9-1,ncbiNodeId:9821,antigenRange:1-163,antigenLength:163,antigenImageFileName:MA5-51799_CD3_Q9XSJ9-1_House_mouse.svg,antigenImageFileNamePDP:MA5-51799_CD3_Q9XSJ9-1_House_mouse_PDP.jpeg,sortOrder:16}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.