Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Keep as concentrated solution.
Predicted reactivity: Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Positive Control: 293T, A549, NIH-3T3, JC, BCL-1, C2C12, Raw264.7.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the function of this modification is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, inhibiting incorporation into microtubules.
Microtubules, major cytoskeletal elements in all eukaryotic cells, consist of tubulin dimers (alpha and beta, ~55 kDa each). They play key roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support. Because beta-tubulin is ubiquitously expressed, it is frequently used as a loading control in protein detection assays such as Western blotting.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.