Thermo Fisher Scientific N-cadherin Polyclonal Antibody
Thermo Fisher Scientific의 N-cadherin Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 WB, IHC, ICC 등 다양한 응용에 적합합니다. 액상 형태로 항원 친화 크로마토그래피로 정제되었으며, 세포 간 칼슘 의존성 접착 연구에 유용합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human N-Cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.53 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792483 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (81%), Xenopus laevis (89%), Dog (98%), Chicken (94%), Bovine (98%)
- Positive Control: 293T, NT2D1, Mouse brain, Rat brain
- Centrifuge briefly prior to opening the vial.
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It plays a crucial role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions. The function of N-cadherin depends on its association with the actin-cytoskeleton and is mediated through interactions with cytoplasmic catenin proteins. This association is regulated by phosphorylation and dephosphorylation of beta-catenin. N-cadherin consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail, functioning during gastrulation and contributing to left-right asymmetry establishment.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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