Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), Brilliant Ultra Violet 661, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant Violet 605
• Brilliant Violet 711
• FITC
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Yellow 570 / 660 / 755
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Custom conjugation available

Product Specific Information

The WM-53 monoclonal antibody reacts with human CD33 (GP67, P67), a 67 kDa type I transmembrane glycoprotein of the Siglec family. CD33 is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells, showing high specificity to the hematopoietic compartment.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
  • Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye emitting at 660 nm, optimized for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility before use.

Staining Recommendations

When using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Handling and Fixation

• Light Sensitivity: Protect vial and samples from light.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using:
  • IC Fixation Buffer (Product # 00-8222-49)
  • 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD33 is a transmembrane protein of the Siglec family containing ITIM motifs that recruit SHP-1 and SHP-2 phosphatases for signal transduction and receptor regulation. It is expressed on myelomonocytic lineage cells, including granulocyte and macrophage precursors, but not on pluripotent stem cells. CD33 serves as a therapeutic target for acute myeloid leukemia and a marker for distinguishing myelogenous leukemia from lymphoid or erythroid types.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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