Thermo Fisher Scientific Phospho-Rb (Ser780) Recombinant Rabbit Monoclonal Antibody (13H9L5)

Applications

Western Blot (WB)

• Tested Dilution: 1–3 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–2 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

• Predicted to react with mouse, rat, Drosophila, non-human primate, and rabbit based on sequence homology.
• On non-reducing gel: intact IgG appears as ~150 kDa band; upon reduction, generates ~25 kDa light chain and ~50 kDa heavy chain.
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
• Advantages include:
  • Better specificity and sensitivity
  • Lot-to-lot consistency
  • Animal origin-free formulations
  • Broader immunoreactivity due to rabbit immune repertoire

Target Information

Rb is a tumor suppressor nuclear phosphoprotein that binds DNA. It is phosphorylated on serine and threonine residues and forms complexes with SV40 large T antigen, adenovirus E1A, and HPV-16 E. Rb regulates transcription, and loss of function leads to uncontrolled cell growth.
Mutations in the RB gene are associated with cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.