Thermo Fisher Scientific MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H13L17

Immunogen

Recombinant protein corresponding to amino acids 221-471 of human MMP-13 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MMP13,uniProtId:P45452-1,ncbiNodeId:9606,antigenRange:221-471,antigenLength:471,antigenImageFileName:701287_MMP13_P45452-1_Rabbit.svg,antigenImageFileNamePDP:701287_MMP13_P45452-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532459

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.