Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (OTI3G4), TrueMAB
Human beta-catenin 단백질(aa 531–781)에 특이적인 Mouse monoclonal antibody (Clone OTI3G4). Western blot에 최적화. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 내 보관. 세포 접착 및 Wnt 신호전달 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G4 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 531–781 of human beta-catenin |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-catenin is an adherens junction (AJ) protein crucial for cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin, linking cell adhesion complexes to the actin cytoskeleton.
Beta-catenin also associates with signaling pathways involving tyrosine kinases, phosphatases, and Wnt/Wingless, influencing morphogenesis and cell proliferation.
Aberrant beta-catenin activity is implicated in various cancers, including colorectal, ovarian, breast, liver, lung, gastric, prostate, and skin tumors.
In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, stimulating target genes such as cyclin D and c-myc.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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