Thermo Fisher Scientific EphB4 Monoclonal Antibody (VEB4-7E4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:50

-

Inhibition Assays (IA)

1:50

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

VEB4-7E4

Immunogen

The monoclonal antibody VEB4-7E4 recognizes mouse Ephrin type-B receptor 4 (EphB4) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EphB4,uniProtId:P54761-1,ncbiNodeId:10090,antigenRange:1-987,antigenLength:987,antigenImageFileName:MA5-49038_EphB4_P54761-1_Rat.svg,antigenImageFileNamePDP:MA5-49038_EphB4_P54761-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_3091086

Product Specific Information

The monoclonal antibody VEB4-7E4 marks venous endothelial cells, but not arterial endothelial cells in B16 melanoma cells.

Target Information

EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.