Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Blue 690, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

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Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes, HLA-A3.1 and HLA-A3.2, differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes. HLA-A3 is expressed by 15–25% of cancer patients and binds the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells
• Recommended use: 5 µL (0.6 µg) per test in 100 µL final volume
• Suitable cell range: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Conjugate Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing nucleic acid dye structures engineered for optimized fluorescent signatures with reduced spillover and spread.

• Excitation: 494 nm
• Emission: 585 nm
• Laser: 488 nm (Blue)

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Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer of a heavy chain and beta-2 microglobulin. Class I molecules present peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells.

• Heavy chain: ~45 kDa
• Gene contains 8 exons
  • Exon 1: leader peptide
  • Exons 2–3: α1 and α2 domains (peptide binding)
  • Exon 4: α3 domain
  • Exon 5: transmembrane region
  • Exons 6–7: cytoplasmic tail
• Polymorphisms in exons 2 and 3 determine peptide binding specificity.
• Typing for these polymorphisms is used in bone marrow and kidney transplantation.
• Hundreds of HLA-A alleles have been identified.

HLA and MHC antibodies are critical in Immunopeptidomics, aiding in the identification and characterization of neoantigens via LC-MS/MS.

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For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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