Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

추가 형광체 포맷

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody GAP.A3 reacts with the human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family. HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2) differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides on the cell surface to cytotoxic T lymphocytes.

HLA-A3 is expressed by 15–25% of cancer patients and has been shown to bind the tumor-associated antigen hTERT.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested with normal human peripheral blood cells at 5 µL (0.6 µg) per test.
A test is defined as the amount that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).

NovaFluor Compatibility:
Not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.

CellBlox Plus Blocking Buffer:
This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
Add 5 µL per sample to antibody cocktails or single-color controls (100 µL cell sample, 10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

HLA-A is a class I heavy chain molecule forming a heterodimer with beta-2 microglobulin. It presents peptides derived from the endoplasmic reticulum lumen and is expressed on nearly all cells.

The heavy chain (~45 kDa) gene contains 8 exons:

• Exon 1: leader peptide
• Exons 2–3: α1 and α2 domains (peptide binding)
• Exon 4: α3 domain
• Exon 5: transmembrane region
• Exons 6–7: cytoplasmic tail

Polymorphisms in exons 2 and 3 determine peptide-binding specificity. Typing for these polymorphisms is used in bone marrow and kidney transplantation.

HLA and MHC antibodies are essential in immunopeptidomics, aiding neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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