Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific HLA-A3 Monoclonal Antibody (GAP.A3), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The monoclonal antibody GAP.A3 reacts with human major histocompatibility complex (MHC) class I molecule HLA-A3, a member of the HLA-A family.
HLA-A3 exists as two subtypes (HLA-A3.1 and HLA-A3.2), differing at amino acid positions 152 and 156. MHC class I molecules are expressed on all nucleated cells and present intracellular peptides to cytotoxic T lymphocytes.

Expression and Relevance:

• HLA-A3 is expressed by 15–25% of cancer patients.
• Demonstrated to bind the tumor-associated antigen human telomerase reverse transcriptase (hTERT).

Kit Contents:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis of normal human peripheral blood cells.

Applications Tested:

• Pre-titrated for use at 5 µL (0.6 µg)/test in a 100 µL sample volume.
• Suitable for 10⁵–10⁸ cells/test.

NovaFluor Compatibility Notes:

• Not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Recommended: Invitrogen LIVE/DEAD Fixable Dead Cell stains.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample containing 10³–10⁸ cells.

NovaFluor Technology:

• Based on Phiton™ technology utilizing novel nucleic acid dye structures.
• Engineered fluorescent signatures minimize spillover and spread impacts.

Excitation/Emission/Laser:

• Excitation: 639 nm
• Emission: 710 nm
• Laser: 633–640 nm (Red)

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues.
This molecule is a heterodimer consisting of a heavy chain and a light chain (β2-microglobulin).
Class I molecules present peptides derived from the endoplasmic reticulum lumen and are expressed in nearly all cells.

Molecular Details:

• Heavy chain: ~45 kDa
• Gene contains 8 exons
  • Exon 1: leader peptide
  • Exons 2–3: α1 and α2 domains (peptide binding)
  • Exon 4: α3 domain
  • Exon 5: transmembrane region
  • Exons 6–7: cytoplasmic tail
• Polymorphisms in exons 2 and 3 determine peptide binding specificity.

Applications:

• Used in bone marrow and kidney transplantation typing.
• Plays a key role in immunopeptidomics for neoantigen identification via LC-MS/MS.

Note:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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