Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, Biotin
Goat anti-Human IgG Fc Cross-Adsorbed Biotin-conjugated 2차 항체로, 인간 IgG Fc를 특이적으로 인식합니다. ICC/IF, Flow Cytometry, ELISA 등에 사용 가능하며, 높은 특이성과 신호 증폭 효과를 제공합니다. Affinity chromatography로 정제된 액상 제품입니다.
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- A18827
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535604 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of specific targets. Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically generated by immunizing host animals with pooled immunoglobulins from the target species, they can be further purified or modified (e.g., immunoaffinity purification, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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