
Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 염소 유래 항-인간 IgG Fc 2차항체로 Western blot, IHC, ELISA 등에 사용 가능. 고순도 친화 크로마토그래피 정제, 다양한 형광 및 효소 결합 포맷 제공. 교차흡착 처리로 비특이적 반응 최소화.
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Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535600 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse and rabbit serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C.
For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, human IgG F(ab')₂ fragment, or serum proteins from bovine, horse, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility enabling use with many detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
Additional Formats Available
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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