Thermo Fisher Scientific RHEB Monoclonal Antibody (2C11)
RHEB 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot 및 ELISA에 적합. 인간, 마우스, 랫트 반응성. PBS 용액 상태로 제공되며 보존제 없음. -20°C에서 보관하며 동결/해동 반복을 피해야 함.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 3 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2C11 |
| Immunogen | RHEB (AAH16155, 1–184 a.a.) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF RRIILEAEKM DGAASQGKSS CSVM
Target Information
Rheb encodes a member of the small GTPase superfamily and represents a lipid-anchored, cell membrane protein containing five repeats of the RAS-related GTP-binding region.
This protein is crucial for regulation of growth and cell cycle progression through the insulin/TOR/S6K signaling pathway. It possesses GTPase activity, cycling between GDP-bound and GTP-bound forms, and requires farnesylation for functional activity.
Three pseudogenes have been mapped: two on chromosome 10 and one on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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