Thermo Fisher Scientific Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 594 dye

Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye

The Click-iT™ EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays optimized for fluorescence microscopy applications. In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

주요 특징

• Simple — 작동이 간단하며, 빠른 시간 내에 안정적인 결과 제공
• Efficient — 변성 단계나 강한 처리 불필요
• Content-rich results — 세포 형태, 항원 구조, DNA 무결성 보존
• Consistent — 항체 로트에 따른 변동성 없음

Superior Proliferation Methodology

The Click-iT™ EdU Cell Proliferation Kit for Imaging provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine incorporated into DNA during active synthesis. With Click-iT™ EdU, mild fixation and detergent permeabilization are sufficient for the small molecule-based detection reagent to access DNA. This makes the kit easy to use, accurate, and compatible with cell cycle analysis and other intracellular or extracellular targets for content-rich results.

This kit is optimized for fluorescence microscopy applications. Visit the Click-iT™ technology area of Thermo Fisher’s website for kits designed for high-content imaging, fluorescence microplate, or flow cytometry platforms.

Notes

• The Click-iT™ assay can be used on cells in culture or in vivo following EdU administration by feeding or injection.
• Dual pulse experiments with BrdU are possible using anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that tolerate fixation or are designed for fixed cell labeling.

사양 (Specifications)

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