Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 28 publications

Product Specifications

Available Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, uniquely expressed on NK cells (including immature NK cells, DX5⁻ CD3⁻), allowing discrimination between NKT and NK cells (NKp46⁺, CD3⁻).

Unlike many NK markers that also stain NKT cells, staining with 29A1.4 is not strain-specific and has been demonstrated in C57Bl/6, SJL, CBA/CA, and BALB/C strains. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.

• The 29A1.4 antibody activates NK cells in vitro but does not deplete NK cells in vivo.
• Reported application: Flow cytometric analysis.
• Tested on mouse splenocytes; use ≤1 µg/test (100 µL final volume).
• Cell number range: 10⁵–10⁸ cells/test.
• Optimize antibody titration for best performance.

Light Sensitivity: PE-Cyanine7 is sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances the ability of activated NK cells to mediate tumor cell lysis. It consists of two Ig-like domains and is structurally related to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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