Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (9E2), PE-Cyanine7, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 4 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The 9E2 monoclonal antibody reacts with human CD335 (NKp46), a member of the natural cytotoxicity receptor family expressed on NK cells. CD335 plays a role in activating and expanding resting NK cells through interaction with dendritic cells, contributing to the elimination of tumor and virus-infected cells. It also interacts with pancreatic beta cells during type I diabetes development. The 9E2 antibody has been reported to induce cytotoxicity.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test (final volume 100 µL). Cell number may range from 10⁵ to 10⁸ cells/test.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Store up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Excitation/Emission:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor that enhances NK cell-mediated tumor cell lysis. It consists of two Ig-like domains similar to leukocyte immunoglobulin-like receptors (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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