Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:5,000–1:20,000
• Publications: —

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Predicted reactivity: Mouse (95%), Zebrafish (85%), Cat (81%), Pig (89%), Chicken (87%), Bovine (86%).
• Positive Control: NT2D1, IMR32, U87-MG, MCF-7.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules.
Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3.
Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear.
Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and this phosphorylation inhibits tubulin incorporation into microtubules.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and beta. They play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for protein detection assays, such as Western blotting.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.