Thermo Fisher Scientific Cas 9 Polyclonal Antibody, DyLight 680
Streptococcus pyogenes Cas9 단백질을 인식하는 DyLight 680 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA 등에 사용 가능. 높은 특이성과 감도를 제공하며, 형광 검출에 최적화됨. 연구용으로만 사용.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| ELISA | 1:25,000–1:30,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein. |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
The CRISPR-Cas9 system enables site-specific genome editing by using RNA to guide the nuclease to a desired DNA sequence. It can induce indel mutations, sequence insertions, deletions, or genomic rearrangements.
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 is smaller, efficient, and highly specific, recognizing NNGRRT PAM sequences and suitable for compact delivery systems such as AAV vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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