Thermo Fisher Scientific Phospho-PRMT4 (Ser228) Polyclonal Antibody
Phospho-PRMT4 (Ser228) 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot 및 ICC/IF에 적합하며 고순도 친화성 크로마토그래피 정제. PBS 버퍼와 50% 글리세롤로 안정화되어 -20°C 보관.
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Thermo Fisher Scientific Phospho-PRMT4 (Ser228) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:1,000
- Immunocytochemistry (ICC/IF): 1:100–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 228 (V-K-S(p)-N-N) derived from Human CARM1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554391 |
Product Specific Information
- Suggested positive control for Western blot: A431 cells
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, forming methylated arginine derivatives and S-adenosyl-L-homocysteine.
Protein arginine methylation is implicated in:
- DNA packaging
- Transcription regulation
- Signal transduction
- mRNA stability
- Metabolism of nascent pre-RNA
- Transcriptional activation
Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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