Thermo Fisher Scientific Phospho-GRF-1 (Tyr1105) Polyclonal Antibody
Phospho-GRF-1 (Tyr1105) 폴리클로날 항체로 인간, 생쥐, 랫트 시료에 반응합니다. Western blot 및 IHC(P)에서 사용 가능하며, 친화 크로마토그래피로 정제되었습니다. PBS와 글리세롤 용액 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 1105 (N-I-Y(p)-S-V) derived from Human GRF-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554396 |
Product Specific Information
A suggested positive control for Western blot is mouse brain cells.
Target Information
RNA polymerase (pol) III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To maintain rapid growth, cells must produce high levels of Pol III-transcribed RNA, requiring TFIIIB and TFIIIC2 transcription factor complexes.
The TFIIIC2 complex, composed of TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63, is overexpressed in adenovirus-transformed and malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may play a key role in malignant cell deregulation.
The TFIIIB complex includes TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, which are also upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to bind and inactivate TFIIIB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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