Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
Publications: View 5 publications

Product Specifications

Available Conjugated Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available upon request

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 or Leu9. CD7 is a 40 kDa receptor and a member of the immunoglobulin gene superfamily.
The N-terminal amino acid sequence (aa1–107) is highly homologous to Ig kappa light chain sequence, while the carboxyl-terminal region is proline-rich and may form a stalk projecting the Ig domain.

CD7 is expressed on:

• Most immature and mature T lymphocytes
• T cell leukemias
• Natural killer (NK) cells
• A small subset of normal and malignant B cells

Functional Role:
Cross-linking CD7 modulates T cell and NK cell activity, inducing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3′-kinase, and its ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.

A clonogenic subpopulation of CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high levels of CD7 has lymphoid lineage potential, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells.
The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.

It has also been suggested that CD7 cooperates with CD28 during Treg function; mice lacking both CD28 and CD7 show reduced Treg numbers and suppressive activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometry of normal human peripheral blood cells (≤1 µg/test).
A test is defined as the amount of antibody staining cells in 100 µL volume (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member. It contains N-terminal amino acids homologous to Ig kappa light chains and a proline-rich extracellular region forming a stalk.
CD7 is expressed on immature/mature T lymphocytes, T cell leukemias, NK cells, and subsets of B cells.
Cross-linking CD7 enhances T and NK cell activation, and its expression is a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.