Thermo Fisher Scientific IRAK4 Polyclonal Antibody
IRAK4 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 마우스, 랫트 반응성. Western blot, Flow cytometry, Immunoprecipitation에 사용 가능. 단백질 G로 정제된 액상 형태, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides corresponding to residues 38–54 and 120–136 of mouse IRAK4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2126576 |
Product Specific Information
Suggested positive control: antigen standard for IRAK4 (transient overexpression lysate), NIH-3T3 whole cell lysate.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK4 activates NF-kappa-B and MAPK pathways. IRAK4 interacts with and phosphorylates IRAK-1. IRAK4-deficient animals are completely resistant to challenge with LPS. Animals and humans lacking IRAK4 are impaired in their responses to viral and bacterial challenges. Members in the IRAK/Pelle family play a central role in IL-1R/TLR-mediated inflammatory responses and innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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