Thermo Fisher Scientific JAK3 Monoclonal Antibody (OTI2B4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family.
JAK3 is predominantly expressed in immune cells and mediates signal transduction in response to activation of interleukin receptors.
Upon cytokine or interferon stimulation, JAKs become phosphorylated at adjacent tyrosine residues, leading to STAT activation, dimerization, and nuclear translocation.
Activated STAT proteins regulate transcription of numerous genes, including interferon-stimulated genes.
JAK2 is essential for IFN-γ receptor complex initiation, while JAK1 serves as an amplifier. Some evidence suggests that JAK2’s function may be replaced by Tyk2 or JAK3 depending on receptor complex configuration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.