Thermo Fisher Scientific LGMN Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-288 of human Legumain (LGMN) (NP_0055973?) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LGMN,uniProtId:Q99538-1,ncbiNodeId:9606,antigenRange:26-288,antigenLength:433,antigenImageFileName:PA5-87990_LGMN_Q99538-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87990_LGMN_Q99538-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.01 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804568

Product Specific Information

Positive Samples: Mouse lung, Mouse liver; Cellular Location: Lysosome

Immunogen sequence: GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IVVMMYDDIA YSEDNPTPGI VINRPNGTDV YQGVPKDYTG EDVTPQNFLA VLRGDAEAVK GIGSGKVLKS GPQDHVFIYF TDHGSTGILV FPNEDLHVKD LNETIHYMYK HKMYRKMVFY IEACESGSMM NHLPDNINVY ATTAANPRES SYACYYDEKR STYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKTI STMKVMQFQG MKR

Target Information

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.