Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Species Reactivity

• Baboon
• Chimpanzee
• Cynomolgus monkey
• Human
• Non-human primate
• Rhesus monkey

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 506
• FITC
• NovaFluor Blue / Red / Violet / Yellow series
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b, associated with integrin beta2 (CD18), is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for ICAM family members CD54, CD102, CD50, and iC3b, playing key roles in cell-cell and cell-matrix interactions.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells at 5 µL (0.5 µg)/test.

Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be fixed in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in dark conditions with minimal effect on brightness and FRET efficiency.

Excitation: 350 nm
Emission: 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that associates with integrin beta-2 (CD18) to form the CD11b/CD18 complex, also known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating critical adhesion processes.

Expression varies among tissues: 8% of spleen cells, 44% of bone marrow cells, and <1% of thymocytes. Commonly used as a microglial marker in nervous tissue. CD11b expression increases during monocyte maturation and is higher in peritoneal macrophages than splenic macrophages. Dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures.

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