
Norgen Staphylococcus aureus TaqMan PCR Detection Kits
Staphylococcus aureus 검출용 TaqMan PCR 키트로, 유제품 내 병원체 감염 진단 연구에 적합합니다. 프라이머 및 프로브 믹스 포함, 양성·음성 대조군 제공. -20°C 보관 시 2년 안정성 유지. 연구용으로만 사용 가능합니다.
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Norgen Staphylococcus aureus TaqMan PCR Detection Kits
SKU: TM29350
Features and Benefits
- Detection kits for Staphylococcus aureus
- Available in TaqMan format for analysis
Product Overview
Mastitis is the single most costly disease of dairy cattle resulting in reduced milk yield and quality. The inflammation of the udder is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds.
S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005).
S. aureus is a facultatively anaerobic, Gram-positive bacterium. Most strains are catalase-positive and coagulase-positive, forming the basis of traditional identification methods.
TaqMan PCR Kit Options
Staphylococcus aureus TaqMan PCR Kit (100 reactions)
- Ready-to-use format including Master Mix for target and PCR control
- Monitors PCR inhibition and validates assay quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Includes Positive and Negative Controls to confirm reagent integrity
Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls (100 reactions)
- Specific Primer/Probe mix and Positive Control for the target organism
- Includes Nuclease-free water
- Compatible with Norgen’s PCR Master Mix (#28007) or customer-supplied mix
For research use only. Not intended for in vitro diagnostics.
Supporting Data
TaqMan PCR Kit - Figure 1
Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channels indicate successful amplification.
그래프 설명 (OCR 추출 텍스트):
- 상단 그래프: Amplification – FAM channel (Y축: RFU / X축: Cycles)
- 하단 그래프: Amplification – HEX channel (Y축: RFU / X축: Cycles)
두 그래프 모두 증폭 과정을 나타내며, 각각 파란색(FAM)과 초록색(HEX) 채널로 표시됩니다.
TaqMan PCR Kit - Figure 2
Example of TaqMan PCR Negative result. No target DNA detected in FAM channel, but amplification signal from HEX indicates successful PCR.
그래프 설명 (OCR 추출 텍스트):
- 상단: Amplification (FAM channel), RFU vs. Cycles
- 하단: Amplification (HEX channel), RFU vs. Cycles
두 그래프는 각각 FAM과 HEX 채널의 증폭 곡선을 표시합니다.
TaqMan PCR Kit - Figure 3
Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channels detected. It is suggested to repeat sample preparation using the recommended DNA purification kit.
그래프 설명 (OCR 추출 텍스트):
- 상단: Amplification – FAM channel
- 하단: Amplification – HEX channel
두 그래프 모두 X축(Cycles), Y축(RFU)로 구성되어 있으며, 증폭 신호가 나타나지 않는 결과를 보여줍니다.
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without performance loss.
Store all components at -20°C upon arrival. Avoid repeated thawing and freezing (>2×) of the Master Mix and Positive Control. For intermittent use, aliquot reagents before freezing.
Kit Components
| Component | Cat. TM29350 (100 preps) | Cat. TM29310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 × 700 μL | - |
| S. aureus Primer & Probe Mix | 280 μL | 280 μL |
| S. aureus Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Documentation
Protocols
- (TM29350) Staphylococcus aureus TaqMan PCR Kit - Protocol (100 rxns)
- (TM29310) Staphylococcus aureus TaqMan Probe/Primer and Control Set - Protocol (100 rxns)
Application Notes
Safety Data Sheets
- TM29350 - Staphylococcus aureus TaqMan PCR Detection Kit - SDS
- TM29310 - Staphylococcus aureus TaqMan PCR Detection Kit - SDS
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