Thermo Fisher Scientific POLR3A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence:
  MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA VIGILQMICK TCCHIMLSQE EKKQFLDYLK RPGLTYLQKR GLKKKISDKC RKKNICHHCG AFNGTVKKCG LLKIIHEKYK TNKKVVDPIV SNFLQSFETA
• Positive Samples: 293T
• Cellular Location: Nucleus

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR3A is the largest and catalytic core component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs.
It forms the polymerase active center together with the second largest subunit.
A single-stranded DNA template strand is positioned within the central active site cleft of Pol III.
A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site, promoting translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching conformations at each step of nucleotide addition.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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