Thermo Fisher Scientific Leptin Receptor (LEPR)/Ob-R/CD295 Monoclonal Antibody (LEPR, 4301)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature (RT).
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

Target Information

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily that plays an important role in mammalian body weight homeostasis and energy balance.
Activation of OB-R by the adipose tissue-secreted hormone leptin triggers downstream signaling via the JAK/STAT pathway.
Several isoforms of OB-R differ in length and signaling capabilities. The long form is expressed in the choroid plexus and hypothalamus, proposed as centers for satiety and energy expenditure.
Mutations in mouse OB-R (db) or leptin (ob) genes result in early-onset obesity and metabolic abnormalities such as hyperinsulinemia, insulin resistance, and increased adipose tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.