Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1 µg)/test
• View 40 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 506
• FITC
• NovaFluor Blue / Red / Violet / Yellow Series
• PE, PE-Cyanine5, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, mediating cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells; use at 5 µL (1 µg) per test (100 µL final volume).
• Light Sensitivity: Protect vial and samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation/Emission: 488–561 nm / 775 nm; compatible with Blue, Green, and Yellow-Green lasers.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that forms a heterodimer with integrin beta-2 (CD18), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 acts as a receptor for ICAMs, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, playing a key role in immune cell adhesion and migration.

CD11b expression varies across tissues—high in bone marrow cells and peritoneal macrophages, low in thymocytes. It serves as a microglial marker and is associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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